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With reference to Coilin Antibody:
From what I can determine, Coilin antibody is used as an assay dilution, often when determining ANA titer. So, it is a process used by some labs to dilute antibodies to determine the titer.
I found this definition in the medical dictionary:
Coilin is a protein that in humans is encoded by the COIL gene. Coilin protein is one of the main molecular components of Cajal bodies (CBs). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification (a process in cell biology which, in primary transcript RNA is converted into mature RNA) of small nuclear and small nucleolar RNAs. In addition to its structural role, coilin acts as glue to connect the CB to the nucleous. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylationof coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14.
To study CBs, coilin can be combined with GFP (Green Fluorescent Protein) to form Colin-GFP hybrid protein. The hybrid protein can then be used to locate CBs underneath a microscope, usually near the nucleolus of the cell. Other proteins that make up the CB include snRNPs and nucleolar snoRNPs.
Coilin got its name from the coiled shaped of the CB in which it is found. It was first identified using human autoimmune serum.
I hope that this has been somewhat helpful to you. Please let us know if you need anything further. Again, welcome to our family!
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